Armadale Thistle 4 Leith Athletic 2
Broxburn Athletic 2 Kirkcaldy & Dysart 2 a.e.t. (Broxburn won on pens)
Burntisland Shipyard 5 Fauldhouse United 1
Camelon 4 Dunipace 1
Civil Service Strollers 3 Bo'ness Athletic 1
Coldstream 3 Luncarty 1
Craigroyston 0 University of Stirling 3
Crossgates Primrose 1 Thornton Hibs 0
Easthouses Lily MW 3 Dundonald Bluebell 5
Edinburgh College 2 Arniston Rangers 2 a.e.t. (Edinburgh College won on pens)
Edinburgh United 1 Edinburgh University 3
Gretna 2008 1 Dunbar United 2
Haddington Athletic 1 West Calder United 2 a.e.t.
Hawick Royal Albert 0 Blackburn United 2
Heriot-Watt University 2 Inverkeithing Hillfield Swifts 0
Jeanfield Swifts 3 Hill of Beath Hawthorn 2
Kinnoull 1 Gala Fairydean Rovers 1 a.e.t. (Kinnoull won on pens)
Linlithgow Rose 6 Dalkeith Thistle 0
Livingston United 0 Oakley United 2
Lochore Welfare 3 Lothian Thistle HV 2
Newtongrange Star 0 Spartans 2
Ormiston Primrose 0 Peebles Rovers 0 a.e.t (Ormiston won on pens)
Preston Athletic 1 Musselburgh Athletic 2
Pumpherston 1 Sauchie 9
St Andrews United 2 Glenrothes 3
Stoneyburn 2 Berwick Rangers 1
Syngenta 1 Penicuik Athletic 2
Tweedmouth Rangers 4 Edinburgh South 5
Tynecastle 3 Newburgh 0
Vale of Leithen 0 Bo'ness United 2
Whitburn 0 Tranent 3
Whitehill Welfare 3 Kennoway Star Hearts 2